What is DNA microarray technology

In it's broadest term, DNA microarray technology may be defined as a high-throughput and versatile technology used for parallel gene expression analysis for thousands of genes of known and unknown function, or DNA homology analysis for detecting polymorphisms and mutations in both prokaryotic and eukaryotic genomic DNA. CartoonCrazy

However, in its precise and accurate definition DNA microarray is an orderly arrangement of thousands of identified sequenced genes printed on an impermeable solid support, usually glass, silicon chips or nylon membrane.

Each identified sequenced gene on the glass, silicon chips or nylon membrane corresponds to a fragment of genomic DNA, cDNAs, PCR products or chemically synthesised oligonucleotides of up to 70mers and represents a single gene.  Online2pdf

Usually a single DNA microarray slide/chip may contain thousands of spots each representing a single gene and collectively the entire genome of an organism. A schematic diagram of the two most commonly used DNA microarray formats to date.


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