Glass cDNA microarrays

There are currently two platforms/types of DNA microarrays that are commercially available.

1. Glass DNA microarrays which involves the micro spotting of pre-fabricated cDNA fragments on a glass slide.
2. High-density oligonucleotide microarrays often referred to as a "chip" which involves in situoligonucleotide synthesis. 

However, from a manufacturing point of view, there are fundamental differences between the two platforms in regard to the sizes of printed DNA fragments, the methods of printing the DNA spots on the slide/chip, and also the data images generated.

Glass DNA microarrays was the first type of DNA microarray technology developed. It was pioneered by Patrick Brown and his colleagues at Stanford University and is produced by using a robotic device, which deposits (spots) a nanoliter of DNA (50-150 µm in diameter) onto a coated microscope glass slide surface in serial order with a distance of approximately 200-250 µm from each other, one spot-one gene. These moderate sized glass cDNA microarrays also bear about 10,000 spots or more on an area of 3.6 cm2.


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