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Showing posts from February, 2021
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  Glass cDNA microarrays There are currently two platforms/types of DNA microarrays that are commercially available. 1. Glass DNA microarrays which involves the micro spotting of pre-fabricated cDNA fragments on a glass slide. 2. High-density oligonucleotide microarrays often referred to as a "chip" which involves  in situ oligonucleotide synthesis.  Animedao However, from a manufacturing point of view, there are fundamental differences between the two platforms in regard to the sizes of printed DNA fragments, the methods of printing the DNA spots on the slide/chip, and also the data images generated. Glass DNA microarrays was the first type of DNA microarray technology developed. It was pioneered by Patrick Brown and his colleagues at Stanford University and is produced by using a robotic device, which deposits (spots) a nanoliter of DNA (50-150 µm in diameter) onto a coated microscope glass slide surface in serial order with a distance of approximately 200-250 µm f
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  What is DNA microarray technology In it's broadest term, DNA microarray technology may be defined as a high-throughput and versatile technology used for parallel gene expression analysis for thousands of genes of known and unknown function, or DNA homology analysis for detecting polymorphisms and mutations in both prokaryotic and eukaryotic genomic DNA.  CartoonCrazy However, in its precise and accurate definition DNA microarray is an orderly arrangement of thousands of identified sequenced genes printed on an impermeable solid support, usually glass, silicon chips or nylon membrane. Each identified sequenced gene on the glass, silicon chips or nylon membrane corresponds to a fragment of genomic DNA, cDNAs, PCR products or chemically synthesised oligonucleotides of up to 70mers and represents a single gene.  O nline2pdf Usually a single DNA microarray slide/chip may contain thousands of spots each representing a single gene and collectively the entire genome of an organis